ANTIOXIDANT AND ANTI-HEMOLYTIC CAPACITIES OF EUCALYPTUS CAMALDULENSIS LEAVES

Authors

• Achwaa Chbib, Department of Biological Sciences, Faculty of Science, Beirut Arab University, Beirut, LebanonFollow
• Malak Mezher, Department of Biological Sciences, Faculty of Science, Beirut Arab University, Beirut, LebanonFollow
• Mohamad Nasser, Plateforme de recherche et d’analyse en sciences de l’environnement (EDST-PRASE), Beirut, LebanonFollow
• Akram Hijazi, Plateforme de recherche et d’analyse en sciences de l’environnement (EDST-PRASE), Beirut, LebanonFollow
• Mahmoud I. Khalil, Department of Biological Sciences, Faculty of Science, Beirut Arab University, Beirut, Lebanon / Molecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, EgyptFollow

Keywords

Antioxidant activity, anti-hemolytic activity, erythrocyte, Eucalyptus camaldulensis, medicinal plant

Disciplines

Life Sciences

Abstract

The effects of medicinal plants on the prevention of many diseases are due to their chemical composition. Eucalyptus camaldulensis (E. camaldulensis) is a native tree in Australia. Its extracts and essential oils (EOs) are applicable in the domains of pharmaceuticals and medicine. This work determined the anti-hemolytic and antioxidant capacities of the ethanolic (EE), methanolic (ME), and aqueous (AE) extracts from the leaves of Lebanese E. camaldulensis.  The leaves of E. camaldulensis were extracted with ethanol, methanol, and water solvent. Qualitative phytochemical analyses were applied to reveal the compounds of these extracts. The Folin-Ciocalteau and Aluminum chloride (AlCl₃) assays were used to estimate the total phenolic content (TPC), the total flavonoid content (TFC), and the total tannin content (TTC). Fourier transform infrared (FTIR) spectroscopy was applied to estimate the chemical composition of the extracts. The 2,2-diphenyl-1-picrohydrazyl (DPPH) radical scavenging assay was used to determine the antioxidant activity of extracts. The hemolytic activity assay and the anti- hemolytic activity assay were used to reveal any damage of extracts on human erythrocytes.  Qualitative phytochemical investigation of extracts revealed the presence of various compounds in the EE, ME, and AE. The quantitative analysis showed the values of the TPC ranging between 228.4 – 363.9 mg GAE/g, the TFC values ranging between 10 – 24.2 mg RE/g, and the TTC values ranging between 49.9 – 76.3 mg GAE/g. Furthermore, FTIR analysis revealed the presence of different functional groups. The DPPH radical scavenging assay showed an antioxidant activity exceeding 90 % for extracts. In addition, these extracts showed that did not exhibit a significant hemolytic effect but they had a significant percentage of anti-hemolysis reaching 90 %.  These results may point to the potential anti-hemolytic and antioxidant capacities of the EE, ME, and AE of E. camaldulensis.

Author ORCID Identifier

Achwaa Chbib www.orcid.org/0009-0005-5168-1488

Malak Mezher www.orcid.org/0009-0000-9176-2091

Akram Hijazi www.orcid.org/0000-0003-4984-8531

Mahmoud I. Khalil www.orcid.org/0000-0001-7629-4357

Recommended Citation

Chbib, Achwaa; Mezher, Malak; Nasser, Mohamad; Hijazi, Akram; and Khalil, Mahmoud I. (2026) "ANTIOXIDANT AND ANTI-HEMOLYTIC CAPACITIES OF EUCALYPTUS CAMALDULENSIS LEAVES," BAU Journal - Science and Technology: Vol. 7: Iss. 2, Article 2.
DOI: https://doi.org/10.54729/2959-331X.1183

ISSN

2959-331X