DEVELOPMENT OF AN INJECTABLE CHITOSAN-BASED BIOINK FOR ENHANCED BONE FRACTURE HEALING VIA MSC-DERIVED OSTEOGENESIS

Authors

Zeina H. Adel, Molecular Biotechnology Program, Faculty of Advanced Basic Sciences, Alamein International University, EgyptFollow
Jana A. Hassan, Molecular Biotechnology Program, Faculty of Advanced Basic Sciences, Alamein International University, EgyptFollow
Hala H. Muhammed, Molecular Biotechnology Program, Faculty of Advanced Basic Sciences, Alamein International University, EgyptFollow
Haneen M. Elsaeid, Molecular Biotechnology Program, Faculty of Advanced Basic Sciences, Alamein International University, EgyptFollow
Raghad M. Nasr, Molecular Biotechnology Program, Faculty of Advanced Basic Sciences, Alamein International University, EgyptFollow
Ahmed M. Deghidy, Molecular Biotechnology Program, Faculty of Advanced Basic Sciences, Alamein International University, Egypt - Molecular Biology Unit, Zoology Department, Faculty of Science, Alexandria University, EgyptFollow

Keywords

Bone Mesenchymal Stem Cells (BM-MSCs), Chitosan, Polyvinyl Alcohol (PVA) Hydrogels, Bioink, Bone Fracture

Disciplines

Animal Experimentation and Research | Cell Biology | Other Cell and Developmental Biology

Abstract

Worldwide, bone fractures are a major public health concern and, there is an urgent requirement to create new treatments to accelerate the rate of the bone healing process. This research mainly relies on creating a hydrogel that satisfies the bone marrow mesenchymal stem cells (BM-MSCs) and makes it a suitable medium to differentiate into osteogenic cells. We combine the BM-MSCs with a chitosan polymer-based hydrogel, infused with PVA to enhance bone healing, this unique mixture forms " Bioink ". We induced a closed fracture model in the rat's right humerus, followed by injection of a single dosage of bioink. On day 14 with the weekly x-ray examination, we noticed complete bone closure although it was expected that for instance, adolescent rats, specifically those aged 6 weeks, are capable of forming bone to bridge the fracture gap within four weeks, so we terminated the experiment on day 14 as bioink, as observed, had reduced the healing time by half, which is a promising result. In conclusion, the current study's findings as a pilot test point out the possibility of this Bioink being involved in the treatment and acceleration of bone fractures.

Author ORCID Identifier

Adel, Zeina H. - https://orcid.org/0009-0004-7631-9816

Hassan, Jana A. - https://orcid.org/0009-0004-8365-3548

Muhammed, Hala H. - https://orcid.org/0009-0001-3278-6461

Elsaeid, Haneen M. - https://orcid.org/0009-0008-0317-4440

Nasr, Raghad M. - https://orcid.org/0009-0005-9915-5196

Deghidy, M Ahmed - https://orcid.org/0009-0001-7428-8081

Recommended Citation

Adel, Zeina H.; Hassan, Jana A.; Muhammed, Hala H.; Elsaeid, Haneen M.; Nasr, Raghad M.; and Deghidy, Ahmed M. (2024) "DEVELOPMENT OF AN INJECTABLE CHITOSAN-BASED BIOINK FOR ENHANCED BONE FRACTURE HEALING VIA MSC-DERIVED OSTEOGENESIS," BAU Journal - Science and Technology: Vol. 6: Iss. 1, Article 3.
DOI: https://doi.org/10.54729/2959-331X.1146

ISSN

2959-331X