OPTIMIZATION OF THE BIO-SYNTHESIS OF MAGNESIUM NANOPARTICLES FROM STAPHYLOCOCCUS HAEMOLYTICUS: A PILOT STUDY

Authors

Mariam Bassam, Department of Biiological Sciences, Faculty of Science, Beirut Arab University, Debbyeh, LebanonFollow
Malak Mezher, Department of Biological Sciences, Faculty of Science, Beirut Arab University, Debbyeh, LebanonFollow
Mahmoud khalil, Department of Biological Sciences, Faculty of Science, Beirut Arab University, Debbyeh, LebanonFollow

Keywords

Nanoparticles, magnesium, Staphylococcus hameolyticus, temperature, metal concentration, incubation time, UV-vis

Disciplines

Architecture | Biology | Business | Engineering | Physical Sciences and Mathematics

Abstract

Nanotechnology is developing rapidly. This field has many influences in humans’ life. Nanoparticles (NPs) have many unique properties including the size, shape, morphology, and surface area. The synthesis of NPs could be achieved by three ways: physical, chemical, and biological. However, the biological synthesis of NPs has a priority on the other domains due to its safety and environmental friendship. In this regard, the main objective of this study was to synthesize magnesium (Mg) NPs from the bacterium S. haemolyticus which was isolated from the Lebanese wastewater. Different parameters were applied to detect the best conditions to produce the highest yield of the target NPs. The effect of the concentration of Mg nitrate solution was studied by applying 5 different concentrations (1, 2, 3, 4, and 5 mM). The effect of the concentration of bacterial culture was detected by applying 3 different concentrations (10⁴, 10⁸, and 10¹² CFU/mL). In addition, the effect of the time of shaking incubation was revealed by applying four different timing (6, 24, 48, and 72 h). The characterization of the NPs was done by Ultraviolet-Visible spectroscopy (UV-Vis) in the range of 200 – 700 nm. The results revealed that the best Mg nitrate solution concentration is 3 mM, the best bacterial culture concentration is the standard 10⁸ CFU/mL (0.5 McFarland), the best shaking incubation time is 48 h, and the best temperature is 37 ˚C. The UV spectra peaks were observed between 250 – 300 nm. The highest peak was observed at 275 nm. The highest absorbance recorded was 0.9 a.u. for the sample of 3 mM Mg nitrate concentration, 10⁸ CFU/mL bacterial concentration, 37 ˚C temperature, and 48 h of incubation. Finally, after detecting the best conditions for the Mg NPs synthesis, they can be applied in different biological domains like anticancer, antibacterial, and against other different diseases.

Author ORCID Identifier

Mariam Bassam https://orcid.org/0009-0005-4830-5852

Malak Mezher https://orcid.org/0009-0006-3698-9625

Mahmoud Khalil https://orcid.org/0000-0001-7629-4357

Recommended Citation

Bassam, Mariam; Mezher, Malak; and khalil, Mahmoud (2023) "OPTIMIZATION OF THE BIO-SYNTHESIS OF MAGNESIUM NANOPARTICLES FROM STAPHYLOCOCCUS HAEMOLYTICUS: A PILOT STUDY," BAU Journal - Science and Technology: Vol. 5: Iss. 1, Article 4.
DOI: https://doi.org/10.54729/2959-331X.1107

ISSN

2959-331X