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Abstract

Green analytical chemistry is concerned with the development of analytical procedures that minimize consumption of hazardous reagents and solvents, and maximize safety for operators and environment. Chemometrics, considered as green analytical chemistry, have become one of the important mathematical and statistical techniques for the resolution of overlapping spectra of multi component mixtures. This work relates simple, accurate and specific analytical chemometric techniques for the simultaneous determination of a ternary mixture of co-administered cardiovascular drugs (Ticagrelor (TICA), Irbesartan (IRB) and Hydrochlorothazide (HCT)). The different applied Chemometric methods are based on H-point standard addition method (HPSAM) and Double divisor ratio spectra Derivative method (DDRD). The applied methods were compared to Derivative spectrophotometry (First derivative (D1) and second derivative (D2)), and shows their superiority in resolving the ternary mixture. TICA, IRB and HCT were determined simultaneously at concentration ratios varying from 0.5: 4: 12.5 µg.mL-1 to 1: 8: 25 or from 10:1:10 to 20:3:8 µg.mL-1, by applying HPSAM or DDRD respectively in a mixed sample. The methods were validated in terms of linearity, LOD, LOQ, precision and accuracy and the results were statistically compared to an established RP-HPLC method.

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