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Abstract

Acute kidney injury (AKI) is a clinical syndrome with diverse pathophysiology. It is associated with increased mortality rate and hospitalization time. Different biomarkers are used for early diagnosis of AKI including Kidney injury molecule-1 (KIM-1). The aim of this study is to determine the association between KIM-1 gene polymorphism and AKI in Lebanese hospitalized patients. Blood samples were collected from fifty AKI patients and forty controls from the Beqaa and North Lebanon to isolate the genomic DNA. Polymerase chain reaction (PCR) was used to amplify exon 4. The amplified PCR products were sequenced. Eleven variations were identified in exon 4 that showed significant association with susceptibility to AKI (P ≤ 0.05). Data analysis suggested that carriers of the risk allele of 5 SNPs had an increased predisposition to the disease. Only 3 SNPs out 11 showed association with serum parameters; rs184276926 and rs10068551 were significantly associated with increased creatinine and urea levels while rs764873440 was associated with urea level only. On the other hand, no association was observed between AKI susceptibility and several clinical parameters. Haplotype analysis showed significant association of 3 haplotype blocks with AKI incidence but not with creatinine/urea levels. In conclusion, our findings showed an association between KIM-1 exon 4 polymorphisms and susceptibility to acute kidney injury.

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