ARUM PALAESTINUM EXTRACTS: A NATURAL SOLUTION FOR ROOT-KNOT NEMATODE CONTROL

Authors

Samaher Ghaith, Department of Biological Sciences, Faculty of Science, Beirut Arab University, Debbyeh, LebanonFollow
Michel Afram, Lebanese Agriculture Research Institute (LARI), Tel Amara, LebanonFollow
Jamilah Borjac, Department of Biological Sciences, Faculty of Science, Beirut Arab University, Debbyeh, LebanonFollow

Keywords

Arum palaestinum, Root knot nematodes (RKN), Meloidogyne spp., Juveniles (J2s), nematicidal activity, egg hatching, gall index

Disciplines

Agriculture | Plant Sciences

Abstract

Root-knot nematodes (RKNs), Meloidogyne spp., have a destructive effect on a wide range of the crops. Arum palaestinum is a wild plant in levant that has a lot of different phytochemicals. The aim of this study is to evaluate the nematicidal potential of A. palaestinum extracts against RKN. The nematicidal efficiency of three types of extracts (aqueous, ethanolic and chloroform) of leaves, flowers and rhizomes of A. palaestinum on second stage juveniles (J2s) and hatching egg rate (RHI) of RKNs were evaluated in vitro using 6 concentrations of each extract (50mg/mL, 37.5mg/mL, 25mg/mL, 12.5mg/mL, 5mg/mL and 2.5mg/mL) and compared to water and dimethyl sulfoxide (4% DMSO) used as negative controls. In general, the aqueous and ethanolic extracts of leaves, flowers, and rhizomes of A. palaestinum had the highest mortality and the hatching inhibition rates followed by the chloroform extracts. Furthermore, the LC50 values determined ranged between 20.76 and 22.31 mg/mL for aqueous extracts, between 21.28 and 21.72mg/mL for ethanolic extracts and between 32.55 to 33.92 mg/mL for chloroform extracts. In the greenhouse experiment, A. palaestinum extracts were added in pots of tomato plants to test the effect of the extracts on the RKN, all extracts significantly reduced RKNs infestation and gall index of M. incognita. The present study revealed the effectiveness of all tested A. palaestinum extracts to root knot nematodes without the surplus of any chemical.

Author ORCID Identifier

Samaher Ghaith https://orcid.org/0009-0000-5796-4087

Jamilah Borjac https://orcid.org/0000-0002-7722-5617

Recommended Citation

Ghaith, Samaher; Afram, Michel; and Borjac, Jamilah (2025) "ARUM PALAESTINUM EXTRACTS: A NATURAL SOLUTION FOR ROOT-KNOT NEMATODE CONTROL," BAU Journal - Science and Technology: Vol. 6: Iss. 2, Article 2.
DOI: https://doi.org/10.54729/2959-331X.1147

ISSN

2959-331X