QUANTITATIVE ASSAY OF BINARY AND TERNARY CARDIOVASCULAR MIXTURES USING ABSORPTION SUBTRACTION SPECTROPHOTOMETRIC METHOD
Binary, ternary, cardiovascular mixtures, absorption subtraction method, multicomponent Formulation, Validation
Other Pharmacy and Pharmaceutical Sciences
Objective: Development and validation of specific spectrophotometric analytical method for the simultaneous determination of co-administered cardiovascular mixtures consisting of Aspirine (ASP) and Prasugrel (PRA) binary mixture and Ticagrelor (TICA), Irbesartan (IRB) and Hydrochlorothazide (HCT) ternary mixture.
Methods: The proposed spectrophotometric method was based on Absorbance Subtraction, where, when a mixture of two drugs X and Y having overlapped spectra, intersecting at isoabsorptive point and Y spectrum is extended more than X, while X doesn’t show any absorbance (A2) at another wavelength (λ2), X and Y could be determined in the mixture by simple mathematical manipulation
Results: This method obeyed Beer’s law in the concentration range of 5-50 μg/ml for ASP, 5-25 μg/ml for PRAS, 10-30 μg/ml for TICA, 0.5-2 μg/ml for IRB, 4-10 μg/ml for HCT. The suggested method was applied on laboratory prepared mixtures and was successfully applied in quality control analysis of combined pharmaceutical dosage forms. The developed spectrophotometric method was validated statistically and recovery studies were carried out, where excellent percentage recoveries were obtained. The developed spectrophotometric method was also compared to reference HPLC methods.
Conclusion: The developed spectrophotometric method based on absorption subtraction is an easy, simple and time saving method, that was able to determine the active ingredients in the binary and ternary pharmaceutical formulation without any interference from then excipients.
El Jamal, Marwa Khaled
"QUANTITATIVE ASSAY OF BINARY AND TERNARY CARDIOVASCULAR MIXTURES USING ABSORPTION SUBTRACTION SPECTROPHOTOMETRIC METHOD,"
BAU Journal - Health and Wellbeing: Vol. 3
, Article 8.
Available at: https://digitalcommons.bau.edu.lb/hwbjournal/vol3/iss1/8